Journal:

Article Title: Sequence Variation in the ftsZ Gene of Bartonella henselae Isolates and Clinical Samples

doi:

Figure Lengend Snippet: Nucleotide sequences of 16S rRNAs and ftsZ genes amplified from the B. henselae isolates studied

Article Snippet: The bacterial strains were grown for 2 weeks on Columbia blood agar containing 5% whole horse blood at 35°C in 5% CO 2 . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate Species Strain Source Geographical origin Additional strain designation Reference 16S rRNA type Bh ftsZ variant B. henselae Houston-1 HIV a patient's blood United States ATCC 49882 22 I 1 3508 B. henselae SA-2 CSD pat United States R1330 I 1 3509 B. henselae CA-1 HIV patient's blood United States R1173 I 1 3507 B. henselae Tiger 2 Cat blood United States R1556 II 1 3750 B. henselae Houston-2 United States I 1 3883 B. henselae Patient's blood United States I 1 3884 B. henselae Patient's blood United States I 1 4271 B. henselae Cat blood United States I 1 4272 B. henselae Cat blood United States I 1 5249 B. henselae United States II 2 URLLY8 B. henselae Marseille Endocarditis patient's blood France R9064 8 II 2 B. henselae FR97/K7ITS Cat blood Germany {"type":"entrez-nucleotide","attrs":{"text":"R14472","term_id":"767548","term_text":"R14472"}} R14472 27 I 1 B. henselae FR96/BK77 Cat blood Germany {"type":"entrez-nucleotide","attrs":{"text":"R14473","term_id":"767549","term_text":"R14473"}} R14473 25 II 3 B. henselae FR96/BK3 Cat blood Germany {"type":"entrez-nucleotide","attrs":{"text":"R14476","term_id":"767552","term_text":"R14476"}} R14476 25 II 3 B. henselae FR96/BK78 Cat blood Germany {"type":"entrez-nucleotide","attrs":{"text":"R14479","term_id":"767555","term_text":"R14479"}} R14479 25 II 3 Open in a separate window a HIV, human immunodeficiency virus.

Techniques: Amplification, Variant Assay

Journal: International Journal of Molecular Sciences

Article Title: Plastid Genomes of the Early Vascular Plant Genus Selaginella Have Unusual Direct Repeat Structures and Drastically Reduced Gene Numbers

doi: 10.3390/ijms22020641

Figure Lengend Snippet: Plastome information about the 19 lycophyte species examined in this study.

Article Snippet: An analysis of the 3507 plastome sequences from algae to seed plants at the National Center for Biotechnology Information (NCBI) as of November 2019 [ ] revealed an average GC content of 37.38 ± 2.26%.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Plastid Genomes of the Early Vascular Plant Genus Selaginella Have Unusual Direct Repeat Structures and Drastically Reduced Gene Numbers

doi: 10.3390/ijms22020641

Figure Lengend Snippet: Phylogenetic tree of five Selaginella tamariscina plastid genome sequences with Selaginella stauntoniana as the outgroup. Phylogenetic analysis was performed with protein coding sequences that all six plastome sequences share with BEAST v2.5.2 software. The HYK substitution model and a gamma category count of 4 were used in the Gamma Site Model. For this analysis, a strict clock with a clock rate of 1, as well as the coalescent constant population tree prior, were applied. 2.66 mya, representing the split between S. tamariscina and S. stauntoniana derived from the phylogenetic analysis in this study , was used as the secondary calibration value with a normal distribution. The five S. tamariscina species were grouped into one monophyletic group, and the chain length was set to 1 M, sampling every 1000 chains. All other values were left at default parameters. Results were checked for the effective sample size (ESS) via Tracer v1.7.1, and the maximum clade credibility tree was drawn by TreeAnnotator with a burnin of 15%. Visualization of the final tree was conducted with FigTree v1.4.4. Blue bars show node bars representing the height 95% HPD, and numbers near the nodes show the median values.

Article Snippet: An analysis of the 3507 plastome sequences from algae to seed plants at the National Center for Biotechnology Information (NCBI) as of November 2019 [ ] revealed an average GC content of 37.38 ± 2.26%.

Techniques: Software, Derivative Assay, Sampling